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Experimental design (see Materials and Methods). Trained and Untrained rats that received neonatal spinally cord transection were used in three experiments: (1) HPLC to measure glutamate and glycine levels; (2) immunohistochemical experiments for VGLUT1 and GLYT2 expression; and (3) hindlimb kinematic analyses. Asterisk (*) denotes that Normal rats were also included for the HPLC experiment (n = 4) and for the kinematic analyses experiment (n = 9).
