TABLE 1.

Rad50 Nucleotide affinities measured by fluorescence anisotropy

ND, not determined.

	KD,ATP	KD,ADP	KI-ATP,ATP	KI-ATP,ADP
	μm	μm	μm	m
L1
Mg 2+	1.51 ± 0.20	0.835 ± 0.23
Mn2+	1.45 ± 0.16	1.67 ± 0.66
No2+ ion	ND	ND
L4
Mg2+	1.94 ± 0.10	0.254 ± 0.043	4.1 ± 0.40	1.5 ± 0.24
Mn2+	2.46 ± 0.18	0.646 ± 0.19	5.7 ± 0.90	2.0 ± 0.30
No2+ ion	ND	1.53 ± 0.41
L1-MRE11
Mg2+	1.86 ± 0.26	0.510 ± 0.089
Mn2+	1.33 ± 0.19	0.840 ± 0.20
No2+ ion	ND	ND
L4-MRE11
Mg2+	1.05 ± 0.12	0.260 ± 0.051	1.1 ± 0.20	1.9 ± 0.28
Mn2+	0.853 ± 0.063	0.280 ± 0.069	0.9 ± 0.13	1.0 ± 0.15
No2+ ion	ND	ND
FL
Mg2+	1.61 ± 0.37	0.328 ± 0.083
Mn2+	1.01 ± 0.41	0.297 ± 0.12
No2+ ion	ND	ND