TABLE 2.
Comparison of the N-terminal amino acid sequences of E. coli CcmE and H. thermophilus cytochrome c552 with the sequencing data (in bold) from the protein complex obtained after incubation of Holo-CcmE* with H. thermophilus apocytochrome c552in vitro for 20 h
Yields are given in picomoles.
| Protein | Amino acid sequence at the N terminus | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CcmE* | M | D | S | N | I | D | L | F | Y | T | |
| Cytochrome c552 | M | N | E | Q | L | A | K | Q | K | G | |
| Protein complex | M | D | S | N | I | D | L | F | Y | T | |
| Yield | 3.2a | 1.8 | 1.7 | 1.8 | 1.7 | 1.5 | |||||
| M | N | E | Q | L | A | K | Q | K | G | ||
| Yield | 1.8 | 1.8 | 2.0 | 1.6 | 1.5 | ||||||
a Indicates the first cycle of sequencing and represents the combined yield of both proteins since Met is the first residue in each.