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. 2011 Nov 28;287(4):2353–2363. doi: 10.1074/jbc.M111.280974

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Fgf9 regulates the gene expression of Pitx2. A, quantitative RT-PCR analysis of Fgf9 and Pitx2 after treatment (+) or no treatment (−) with p38 MAPK inhibitor of primary MEPM cells from Tgfbr2^fl/fl control (open columns) and Tgfbr2^fl/fl;Wnt1-Cre (closed columns) mice. *, p < 0.05. B, quantitative RT-PCR analysis of Fgf9 and Pitx2 after treatment (+) or no treatment (−) with p38 MAPK inhibitor in organ culture of E14.5 palatal mesenchyme from Tgfbr2^fl/fl control (open columns) and Tgfbr2^fl/fl;Wnt1-Cre (closed columns) mice. *, p < 0.05. C and D, gene expressions of Fgf9 (C) and Pitx2 (D) after siRNA knockdown of Fgf9 in primary MEPM cells from Tgfbr2^fl/fl mice. *, p < 0.05. Antisense siRNA treatment was used as control (Ctrl). *, p < 0.05. E and F, gene expression of Fgf9 (E) and Pitx2 (F) after siRNA knockdown for Pitx2 in primary MEPM cells from Tgfbr2^fl/fl mice. *, p < 0.05. Antisense siRNA treatment was used as control (Ctrl). Error bars, S.D.