FIGURE 6.

Effect of NOS inhibitors and exogenous NO and NMDAR antagonists on the JNK downstream mitochondrial signaling pathway. A, C, E, and G, rats were treated with 7-NI, AMT, SNP, or MK801 to observe their effects on the JNK downstream mitochondrial signaling pathway proteins and on the apoptotic response induced by transient brain ischemia followed by 6 h of reperfusion in the hippocampal CA1. Protein samples were separately examined by immunoblotting (IB) with anti-p-Bcl-2, anti-Bcl-2, anti-Cyt c, anti-COX IV, anti-caspase-3, and anti-actin antibodies. B, D, F, and H, Western bands were scanned, and the intensities are represented as the fold changes versus the sham treatment. Data are the means ± S.D. from four independent experiments. a, p < 0.05 versus the sham group; b, p < 0.05 versus the saline group.