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. 2011 Dec 5;287(4):2437–2445. doi: 10.1074/jbc.M111.315051

FIGURE 3.

FIGURE 3.

APP expression is not induced in activated astrocytes in vitro and in vivo. A, 12 DIV primary hippocampal cultures were treated with PBS (Control), 10 ng/ml TNFα, and 50 ng/ml TNFα followed by immunocytochemical staining for APP. Blue, DAPI; Green, GFAP; Red, Y188. Scale bar, 10 μm. B, controlled cortical impact-induced TBI was performed on adult mouse brain. APP immunostaining was carried out in uninjured controls (Control), 24 or 72 h after TBI. Blue, DAPI; Green, GFAP; Red, Y188. Scale bar, 50 μm. C, immunostaining of 20 month-old APP/hAβ/PS1 brains. Upper panel, APP detected by Y188 (red) was found around 4G8-positive Aβ plaques (green). Lower panel, GFAP-positive reactive astrocytes (green) do not express APP (red). Scale bar, 50 μm.