FIGURE 4.

Impaired generation of μHC⁺λ5⁺ cells and pre-BCR⁺CD79b^low in Fut8^−/− CD19⁺CD43⁻ pre-B cells. CD19⁺CD43⁻ pre-B cells (R2) and CD19⁺CD43⁺ pro-B cells (R1) were gated. Upper panels indicate Fut8^+/+ cell subsets and lower panels indicate Fut8^−/− cell subsets. Numbers adjacent to the boxed areas indicate the frequency of μHC⁺λ5⁺ cells and pre-BCR⁺CD79b^low cells in each as a percentage of the total cells. Data were representative of the mean ± S.D. of 3 per genotype (**, p < 0.01). Data were analyzed using the FLOWJO software program.