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. 2011 Nov 14;287(4):2500–2508. doi: 10.1074/jbc.M111.303123

FIGURE 6.

FIGURE 6.

The pre-B colonies in the Complete methylcellulose medium in the presence of SL156 and IL-7. A, comparison of the frequencies of 70Z/3, 70Z/3-KD, and 70Z/3-KD-re cells in response to SL156 + IL-7. B, comparison of the frequencies of pre-B cells between Fut8+/+ CD19lowCD43 and Fut8−/− CD19lowCD43 cells in response to SL156 and IL-7. CD19lowCD43 pre-B cells were isolated using a FACStar Plus (BD Biosciences) instrument. Sorted cell populations were routinely re-analyzed and showed more than 92% purity. The pre-B colonies counted were cultured for 7 days in Complete methylcellulose medium in the presence of SL156 and IL-7. Data are presented as mean ± S.D. of pre-B colony-forming units.