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. 2011 Dec 8;287(4):2666–2677. doi: 10.1074/jbc.M111.246173

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — In vivo STAT3 inhibition showed decreased expression of collagen modulator genes. A, in renal artery-ligated rats (14 days) expression of collagen modulator genes like MMP-2, MMP-9, TIMP-1, TIMP-2, and LOX was significantly higher (second lane (Ligation+DMSO) and third lane (Ligation+NSsiRNA)) compared to sham-treated rats (lane 1) as revealed by RT-PCR. Significant down-regulation in the expression of collagen modulator genes was observed in STAT3 siRNA (fourth lane, Ligation+STAT3siRNA) and S31-201 (fifth lane, Ligation+S31-201)-treated renal artery-ligated rats compared to untreated hypertrophied rats. GAPDH was used as loading control for these experiments. B, graphic representation of the above RT-PCR results shows significant down-regulation of MMP-2, MMP-9, TIMP-1, TIMP-2, and LOX gene expression with STAT3 siRNA and S31-201 treatments (14 days) compared with ligated animals; **, p < 0.01 with respect to Sham-treated; ††, p < 0.01 with respect to ligated (L) or L+NSsiRNA treatment; n = 10 in each group).