FIGURE 1.
Effect of SGK1 on STIM1 and Orai1 transcript levels in HEK293 cells. Shown are the arithmetic means (±S.E., n = 13) of the abundance of mRNA encoding STIM1 (A) and Orai1 (B) in HEK293 cells after transfection with constitutively active SGK1S422D or inactive SGK1K127N with or without treatment with Wogonin (100 μm). * (p < 0.05), ** (p < 0.01), and *** (p < 0.001) indicate statistically significant differences (ANOVA). C, confocal microscopy of p65 (red) translocation in HEK293 cells transfected with active SGK1S422D (green) or inactive SGK1K127N (green) or EGFP-empty vector (green) with or without treatment with Wogonin (100 μm) is shown. For a positive control of p65 translocation, HEK293 cells were incubated with TNFα (100 ng/ml, 1h). Nuclei were stained with DRAQ5 (blue). Arrows indicate transfected cells. The experiment was performed three times.