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. 2011 Nov 21;287(4):2719–2730. doi: 10.1074/jbc.M111.275925

FIGURE 2.

FIGURE 2.

Effect of SGK1 and NF-κB on STIM1 and Orai1 transcript levels in BMMCs. A, confocal microscopy of p65 (red) translocation in BMMCs from gene- targeted mice lacking functional SGK1 (sgk1−/−) and their wild type (sgk1+/+) littermates with or without (control) exposure to SCF (100 ng/ml) is shown. Nuclei were stained with DRAQ5 (blue). B, arithmetic means (±S.E., n = 14) of relative mRNA levels of STIM1 (left panel) and Orai1 (right panel) in sgk1+/+ and sgk1−/− BMMCs with or without exposure to SCF (100 ng/ml) and with or without the presence of Wogonin (100 μm) are shown. Knock out of SGK1 was determined utilizing RT-PCR and agarose gel electrophoresis. ** (p < 0.01) and *** (p < 0.001) indicate statistically significant differences in one group, and # (p < 0.05) and ### (p < 0.001) indicate statistically significant differences between groups (ANOVA). C, arithmetic means (±S.E., n = 6) of relative mRNA levels for STIM1 (left and middle left panel) and Orai1 (right and middle right panel) in BMMCs from sgk1−/− and sgk1+/+ mice without (control) or with transfection with a constitutively active p65 mutant are shown. The success of transfection was determined utilizing RT-PCR and agarose gel electrophoresis. * (p < 0.05) and **(p < 0.01) indicate statistically significant differences (Student's unpaired t test).