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. 2011 Nov 21;287(4):2719–2730. doi: 10.1074/jbc.M111.275925

FIGURE 3.

FIGURE 3.

Effect of the NF-κB subunits p65, p50, and p52 on STIM1 and Orai1 transcript levels in HEK293 cells. Arithmetic means (±S.E., n = 14–17) of the abundance of mRNA encoding STIM1 (A) and Orai1 (B) in HEK293 cells expressing the NF-κB subunits p65/p50 or p65/p52. C, the success of NF-κB subunit transfection was determined utilizing RT-PCR and agarose gel electrophoresis. Upper left panel, shown is a representative agarose gel of cDNA after PCR extracted from HEK293 cells transfected with p65/p50 or p65/p52. TBP was used as an internal control. Upper right and lower panel, shown are the arithmetic means (±S.E., n = 14–17) of the mRNA abundance encoding p65 (upper right panel), p50 (lower left panel), or p52 (lower right panel) in HEK293 expressing p65/p50 or p65/p52 as analyzed by the 2−ΔΔCT method (27). * (p < 0.05) and ** (p < 0.01) indicate statistically significant differences (Student's unpaired t test or ANOVA).