Colocalization of NEIL2 and PNKP in mitochondria. The mitochondrial presence of NEIL2 (A) and PNKP (B) was investigated in human neuroblastoma SH-SY5Y cells using Abs for NEIL2 (rabbit polyclonal; see Ref. 5), PNKP (rabbit polyclonal), and mitochondrion-specific MT-CO2 (mouse monoclonal; Santa Cruz Biotechnology). The nuclei are counterstained with DAPI (blue). Red, NEIL2 and PNKP; green, Mitochondria; yellow, overlap of red and green showing significant co-localization of NEIL2 and PNKP with MT-CO2. Other details are under “Experimental Procedures.”