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. 2011 Dec 5;287(4):2896–2906. doi: 10.1074/jbc.M111.297028

FIGURE 1.

FIGURE 1.

hnRNPA2/B1 is up-regulated during SMC differentiation. A, Hnrnpa2/b1 mRNA was up-regulated during SMC differentiation. Undifferentiated ES cells were plated onto dishes coated with 5 μg/ml collagen IV and cultured in DM. Total RNA from undifferentiated ES cells (day 0) or differentiating ES cells at days 3, 5, and 7 were harvested and subjected to RT-qPCR analysis with primers specific for Hnrnpa2/b1. B, nuclear protein from undifferentiated ESCs (day 0) or differentiating ES cells at the indicated time points were harvested and subjected to Western blot analysis. Upper panels show the representative images, and bottom panel show bar graphs representing means ± S.E. of densitometric analysis (n = 3) of the relative protein levels of hnRNPA2B1. C, nuclear localization of hnRNPA2/B1 in differentiating ES cells. Immunofluorescence staining was conducted on day 7 differentiating ES cells with antibodies against hnRNPA2/B1. The data presented here are representative or an average of three independent experiments. Significant difference from control, *, p < 0.05.