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. 2011 Dec 5;287(4):2896–2906. doi: 10.1074/jbc.M111.297028

FIGURE 5.

FIGURE 5.

hnRNPA2/B1 mediates SMC differentiation partially through regulation of Cbx3 gene expression. A, Cbx3 gene activation was essential for hnRNPA2/B1-mediated SMC gene expression. Day 3 differentiating ES cells were co-transfected with control (pCMV5) or hnRNPA2/B1 expression plasmids pCMV5-Hnrnpa2/b1 (2 μg/106 cells) and control random siRNA or Cbx3 siRNAs (100 nm). Total RNA were harvested 72 h after transfection and subjected to RT-qPCR analysis. B, impairment of SMC gene expression mediated by hnRNPA2/B1 knockdown was rescued by Cbx3 overexpression. Day 3 differentiating ES cells were co-transfected with control random siRNA or Hnrnpa2/b1 siRNAs (100 nm) and control (pCMV5) or Cbx3 expression plasmids pCMV5-Cbx3 (2 μg/106 cells). Total RNA was harvested 72 h after transfection and subjected to RT-qPCR analysis. The data presented here are a representative or mean ± S.E. of three independent experiments, *, p < 0.05.