FIGURE 7.

Accumulation of [³H]CQ and HCQ in McARH7777 cells and McA-TTP cells. A, cells were preincubated without/with 50 nm bafilomycin A1 (Baf A1) before the addition of [³H]CQ. B, subcellular localization of HCQ in McARH7777 (a and b) and McA-TTP cells (c and d). Cells were treated with 100 μm HCQ for 6 h. Autofluorescence of HCQ (excitation 480 ± 40 nm, emission 527 ± 30 nm) was detected as described previously (13). Bright field image (a and c) and fluorescence image of HCQ (b and d) in same cells. Scale bar = 10 μm.