Table 1.
Bacterial strains and plasmids
| Bacterial strains | Description | Source or reference |
|---|---|---|
| E. coli | ||
| DH5α | supE44 ΔlacU(φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | laboratory stock |
| SCS110 | endA dam dcm supE44 Δ (lac-proAB) (F’traD36 proAB lacIqZΔM15) | laboratory stock |
| Rosetta | F− ompT hsdSB(RB− mB−) gal dcm λ(DE3) pRARE (camR) | Novagen |
| D. radiodurans | ||
| R1 | ATCC 13939 | laboratory stock |
| GY11733 | rpoBΔ1250–1258, [RifR] | [17] |
| GY11944 | R1/p11520 | [38] |
| GY12830 | ddrB::spa::cat | this work |
| GY12835 | ΔddrBΩkan | this work |
| GY12966 | ΔrecOΩhph | [36] |
| GY12968 | ΔrecAΩkan | [36] |
| GY13378 | ΔddrBΩkan/p11520 | this work |
| GY13384 | ΔddrBΩkan/p13421(ddrB+) | this work |
| GY13928 | ddrBΔ5::kan | this work |
| GY13930 | ddrBΔ41::kan | this work |
|
| ||
| plasmids | ||
|
| ||
| p11086 | Source of kanamycin cassette in D. radiodurans | laboratory stock |
| p11520 | Shuttle vector E. coli/D radiodurans, SpcR | laboratory stock |
| P11559 | Shuttle vector E. coli/D radiodurans, SpcR | laboratory stock |
| p12723 | Source of flag-tag chloramphenicol cassette | [19] |
| p13421 | p11520 with a PCR fragment encoding ddrB | this work |
| pET21a | T7 expression vector | Novagen |
| pEAW571 | pET21a (NdeI/HindIII) with a PCR fragment encoding ddrB | [6] |
| pEAW588 | pET21a (NdeI/HindIII) with a PCR fragment encoding ddrBΔ41 | this work |