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. Author manuscript; available in PMC: 2012 Mar 16.
Published in final edited form as: Mol Cell. 2011 Sep 16;43(6):962–972. doi: 10.1016/j.molcel.2011.08.008

Figure 2. Ded1 affects the accumulation of stress granules that contain a subset of translation initiation factors.

Figure 2

A. Plasmids containing Ded1-GFP (pRP1556) or Pub1-mCh (pRP1661) were transformed into wild type yeast (yRP2065) and tested for co-localization with or without glucose. B. Strain yRP2065 was transformed with plasmids containing ded1Δ141-150-GFP (pRP2071) and Edc3-mCh (pRP1574). Foci formation was analyzed as in A. White traces show the outline of a cell. The arrows point to the foci in the inset. C. Strain yRP2065 was transformed with plasmids containing Pab1-GFP (pRP1657) and either an empty vector (pRP245) or wild type (pRP2086) or mutant DED1 (i.e. pRP2118) under a galactose-inducible promoter. Foci formation was assessed after 2 or 4 hours of induction in galactose. The same strains, when grown in sucrose where DED1 is not over-expressed, all resemble the empty vector control (data not shown). D. Yeast strains with GFP integrations in the chromosome (see Table S1) were transformed with either an empty vector (pRP1827) or wild type (pRP1559) or mutant DED1 (pRP1564) under a galactose-inducible promoter. Foci formation was assessed after 4 hours in galactose. E. Wild type DED1 (pRP1559) was over-expressed in yeast and immunoprecipitated under native conditions. RNA associated with Ded1 was extracted and analyzed by Northern blot with an oligo dT(36) probe. See also Figure S3.