Skip to main content
. Author manuscript; available in PMC: 2012 Mar 16.
Published in final edited form as: Mol Cell. 2011 Sep 16;43(6):962–972. doi: 10.1016/j.molcel.2011.08.008

Figure 3. Ded1 represses translation initiation via the assembly domains and prior to 48S accumulation.

Figure 3

A. In vitro translation in yeast extract with capped, polyadenylated luciferase mRNA and increasing amounts of recombinant wild type or mutant Ded1. Translation in the absence of additional rDed1 was normalized to 100. Error bars represent the high and low values of duplicate reactions of a representative experiment. These mutants were tested in parallel at least three times with three independent yeast extract and recombinant protein preparations. B. Yeast extract, capped and polyadenylated radiolabeled MFA2 mRNA, and GMP•PNP were incubated with or without recombinant wild type rDed1 or rded1-E307A before separation by sucrose gradient. Fractions were measured for the presence of radiolabeled mRNA to assess 48S formation. See also Figure S4.