Figure 5.

The 24-nt dme-miR-34 isoform is probably remodeled to shorter isoforms after its loading to Ago1. (A) The miR-34 pre-miRNA produces huge amount of miR-34 isoforms in D. melanogaster, and the 24-nt isoform is the miRBase annotated mature sequence. (B) The hairpin structure of dme-mir-34 shows that only the 24-nt isoform can form the correct duplex with miR-34*. (C) The miR-34 isoforms co-express during D. melanogaster development, but the 21-nt isoform is the highest one. (D) The expression patterns of two miR-34 isoforms in C. elegans are not significantly different. (E) Only 20- to 22-nt dme-mir-34 isoforms are associated with Ago proteins.