Figure 3.

Survivals of C. elegans wild-type strain Bergerac-BO, and two recombinant-congenic lines derived from Bergerac-BO by introgression of stP13–stP35 segments from chromosome IV of CL2a. The lineage designated “4L” retained the Bergerac-BO allele only at marker stP44, while the “lsq4 (center-right)” cohort retained the Bergerac-BO allele for stP13 and stP35. Homozygotes for the introduced lsq4 region were ascertained, expanded, and used to measure survival at 20°C. Procedures for nematode culture, scoring, and survival analysis are described in Experimental Procedures. Each survival began with 60–65 worms in two dishes, cultured on agar medium. Adult age is measured from the L4 larval-stage; total age is the indicated adult age plus 3 days, for Bergerac-BO and strains derived by backcrossing into it.