Table 2. Crystal sensitivity statistics determined following the radiation damage protocol described in this work.
The values are organized by sample and the position (Pos) at which the data were collected. The columns 〈J〉/〈σJ〉i and 〈J〉/〈σJ〉f represent the signal-to-noise ratio in the last resolution shell for the first and last wedges. The last column shows the β value corrected according to RADDOSE calculations using the known chemical composition of the samples. The β values are given in Å2 MGy−1. The standard deviation was calculated according to the formula
=
, where μ is the average value.
| Crystal | Pos | Mosaicity (°) | 〈J〉/〈σJ〉i | 〈J〉/〈σJ〉f | β | βaverage | βstd.deviation | βcorrected |
|---|---|---|---|---|---|---|---|---|
| Thermolysin | 1 | 0.56 | 5.2 | 2.0 | 0.74 | 0.74 | 0.06 | 0.57 |
| 2 | 0.51 | 3.9 | 1.6 | 0.82 | 0.63 | |||
| 3 | 0.53 | 4.6 | 2.3 | 0.67 | 0.52 | |||
| Trypsin | 1 | 0.54 | 12.1 | 5.1 | 0.66 | 0.71 | 0.04 | 0.61 |
| 2 | 0.61 | 13.4 | 5.2 | 0.71 | 0.66 | |||
| 3 | 0.69 | 15.2 | 5.5 | 0.77 | 0.71 | |||
| 4 | 0.63 | 16.8 | 6.3 | 0.72 | 0.67 | |||
| 5 | 0.53 | 13.4 | 5.6 | 0.75 | 0.69 | |||
| 6 | 0.63 | 15.8 | 7.3 | 0.65 | 0.60 | |||
| A-DNA | 1 | 0.32 | 13.3 | 8.1 | 0.80 | 0.82 | 0.03 | 0.61 |
| 2 | 0.40 | 8.6 | 6.6 | 0.81 | 0.62 | |||
| 3 | 0.46 | 8.8 | 7.6 | 0.86 | 0.66 | |||
| FAE 1 | 1 | 0.25 | 9.1 | 3.6 | 0.90 | 0.9 | 0.01 | 0.63 |
| 2 | 0.23 | 9.0 | 3.6 | 0.89 | 0.62 | |||
| 3 | 0.17 | 6.5 | 3.4 | 0.90 | 0.63 | |||
| FAE 2 | 1 | 0.31 | 9.3 | 3.9 | 0.80 | 0.88 | 0.1 | 0.56 |
| 2 | 0.27 | 12.5 | 4.5 | 0.81 | 0.57 | |||
| 3 | 0.25 | 7.4 | 2.1 | 1.02 | 0.71 | |||
| RecR | 1 | 0.88 | 4.3 | 1.9 | 0.86 | 0.78 | 0.08 | 0.76 |
| 2 | 0.90 | 5.1 | 2.7 | 0.68 | 0.60 | |||
| 3 | 0.60 | 4.5 | 1.9 | 0.74 | 0.65 | |||
| 4 | 0.86 | 3.9 | 1.9 | 0.85 | 0.75 | |||
| GPCR | 1 | 0.80 | 15.1 | 6.8 | 0.62 | 0.62 | NA | 0.64 |