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. 2012 Feb 1;23(3):401–411. doi: 10.1091/mbc.E11-02-0095

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© 2012 Silkworth et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Incomplete spindle pole separation at NEB promotes formation of merotelic kinetochore attachments. (A, B) Examples of GFP–γ-tubulin PtK1 cells imaged by time-lapse microscopy from NEB to late prometaphase, then immunostained for KTs and MTs, relocalized, and imaged by confocal microscopy (far right). A cell with complete spindle pole separation at NEB is shown in A, whereas a cell with incomplete spindle pole separation is shown in B. Insets represent 225% enlargements of the KTs indicated by the arrowheads. (C) The histogram shows that prometaphase cells with incomplete spindle pole separation at NEB exhibit higher (t test, p < 0.01) numbers of merotelic KTs than do cells with complete spindle pole separation at NEB. Error bars represent standard errors of the mean. Scale bars in the time-lapse images, 10 μm. Scale bars in the fixed cell images, 5 μm.