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. 2012 Feb 1;23(3):412–422. doi: 10.1091/mbc.E11-07-0631

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© 2012 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Spa2p contributes to cell growth both through binding the SBD of Bni1p and independent of the SBD. (A) Growth assays of class I Bni1p truncations in SPA2 and spa2Δ cells at 26°C on YPD plates. (B) F-Actin staining with phalloidin and GFP localization of the indicated constructs in spa2Δ cells. Scale bar, 2 μm. (C) Growth of spores derived from individual tetrads (arrayed vertically) after sporulation of the indicated diploids. Circles indicate bni1Δ79-988 or bni1Δ79-1230 SPA2 spores; rectangles indicate bni1Δ79-988 or bni1Δ79-1230 spa2Δ spores.