FIGURE 4:

Deletion of N-terminal regions of Bni1p leads to progressive defects in nuclear segregation. (A) Growth of 10-fold serial dilutions of cells with N-terminal deletions in ARP1 (SC-URA plates that retain the CEN-URA3-ARP1 plasmid) and arp1Δ (5-fluoroorotic acid plates that select against the CEN-URA3-ARP1 plasmid) cells grown at 26°C. (B) Growth of 10-fold serial dilutions of cells with class I N-terminal deletions in ARP1 and arp1Δ cells at either at 26 or 14°C on YPD plates or at 26°C in the presence of 10 μg/ml benomyl. (C) Percentage of class I deletions in ARP1 and arp1Δ cells with the indicated number of nuclei in the mother cell. Cells were grown at room temperature until OD = 0.2 and then shifted to 16°C for 8 h, and >500 cells were analyzed following staining with 4′,6-diamidino-2-phenylindole. (D) Percentage of cells with misoriented spindles in small- to medium-budded cells. n > 100 for each sample. (E, F) Growth of 10-fold serial dilutions of cells as indicated under the same conditions used in B.