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. 2012 Feb 1;23(3):412–422. doi: 10.1091/mbc.E11-07-0631

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© 2012 Liu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — The dominant effects of a class II Bni1p deletion are due to mislocalized assembly of actin. (A) Growth of spores derived from individual tetrads (arrayed vertically) after sporulation of the indicated diploids. Circles indicate BNI1 arp1Δ spores; rectangles indicate bni1Δ79-998 arp1Δ or bni1Δ79-998-I1431A arp1Δ spores. (B) Growth of 10-fold serial dilutions of cells with the indicated genotypes on YPD plates at 26 and 14°C. (C) Growth of cells expressing bni1Δ79-988 without or with an appended N-terminal CRIB domain. (D) F-Actin staining with phalloidin and GFP localization of the indicated construct. Scale bars, 5 μm.