TABLE 1:
Localization of Bni1p N-terminal overexpression constructs.
| Bni1p-GFP constructs | Bud tip localization | Crescent at bud cortex | Bud neck localization |
|---|---|---|---|
| Bni1p | +++ | + | +++ |
| 1–1239 | +++ | ++++++ | +++ |
| 1–834 | +++ | +++++ | +++ |
| 1–717 | +++ | +++++ | +++ |
| 822–992 (LD3) | ++ | + | ++ |
| 1–596 | + | + | + |
| 1–596-LZ | +++ | +++++ | +++ |
| 1–333 | − | − | + |
| 1–333-LZ (LD1) | +++ | +++++ | +++ |
| 334–821 (LD2) | +++ | +++ | +++ |
| ΔDD 1–834 | +++ | +++ | +++ |
| 501–717 | − | − | − |
| 592–834 | − | − | − |
| 987–1239 | − | − | − |
Numbers indicate Bni1p amino acid residues present in the constructs. A plus or minus indicates whether the construct was detected by live imaging at the indicated place. The number of plus signs indicates how frequently it was seen in a log-phase culture.