FIGURE 6:

Molecular contacts of YidCΔ307 RNCs in INV and proteoliposomes. (A) Cartoon showing the 98–amino acid long YidCΔ307-RNCs used in this study. YidCΔ307-98 RNCs comprise the first 98 amino acids of YidCΔ307 (Figure 5C) and contains pBpa at position 46 in TM 2. (B) YidCΔ307-98 RNCs were synthesized in the presence of INVs. Cross-linking products were identified by immunoprecipitations after separation on 15% SDS–PAGE. (C) YidCΔ307-RNCs were synthesized in the presence of SRP, purified via a sucrose cushion, and incubated with FtsY and liposomes/proteoliposomes as indicated. Samples were loaded on a 7–18% SDS gel. Cross-links to Ffh (asterisk), YidC (cross), and SecY (triangle) are indicated.