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. 1981 Jun 11;9(11):2589–2598. doi: 10.1093/nar/9.11.2589

Clustering of RNA polymerase B molecules in the 5' moiety of the adult beta-globin gene of hen erythrocytes.

P Gariglio, M Bellard, P Chambon
PMCID: PMC326874  PMID: 6269056

Abstract

Nuclei were prepared from mature and immature hen erythrocytes and incubated for RNA synthesis in the absence or in the presence of Sarkosyl. The in vitro labelled synthesized RNA was hybridized to specific 5' and 3' fragments of the chicken adult beta-globin gene to investigate the possible presence of RNA polymerase molecules bound to this gene in the form of transcriptional complexes. Surprisingly, such RNA polymerase B molecules were found located preferentially in the 5' end moiety of the beta-globin genes of mature erythrocytes, although they are apparently evenly distributed along the beta-globin genes of immature polychromatic erythrocytes. The significance of these observations with respect to (1) preferential DNaseI sensitivity of "genes which have been transcribed" and (2) control of transcription in eukaryotic cells is discussed.

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Selected References

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  1. Alwine J. C., Kemp D. J., Parker B. A., Reiser J., Renart J., Stark G. R., Wahl G. M. Detection of specific RNAs or specific fragments of DNA by fractionation in gels and transfer to diazobenzyloxymethyl paper. Methods Enzymol. 1979;68:220–242. doi: 10.1016/0076-6879(79)68017-5. [DOI] [PubMed] [Google Scholar]
  2. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bellard M., Kuo M. T., Dretzen G., Chambon P. Differential nuclease sensitivity of the ovalbumin and beta-globin chromatin regions in erythrocytes and oviduct cells of laying hen. Nucleic Acids Res. 1980 Jun 25;8(12):2737–2750. doi: 10.1093/nar/8.12.2737. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dick C., Johns E. W. A quantitative comparison of histones from immature and mature erythroid cells of the duck. Biochim Biophys Acta. 1969 Mar;175(2):414–418. doi: 10.1016/0005-2795(69)90020-8. [DOI] [PubMed] [Google Scholar]
  5. Dodgson J. B., Strommer J., Engel J. D. Isolation of the chicken beta-globin gene and a linked embryonic beta-like globin gene from a chicken DNA recombinant library. Cell. 1979 Aug;17(4):879–887. doi: 10.1016/0092-8674(79)90328-3. [DOI] [PubMed] [Google Scholar]
  6. Fraser N. W., Sehgal P. B., Darnell J. E., Jr Multiple discrete sites for premature RNA chain termination late in adenovirus-2 infection: enhancement by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2571–2575. doi: 10.1073/pnas.76.6.2571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gariglio P., Buss J., Green M. H. Sarkosyl activation of RNA polymerase activity in mitotic mouse cells. FEBS Lett. 1974 Aug 30;44(3):330–333. doi: 10.1016/0014-5793(74)81170-1. [DOI] [PubMed] [Google Scholar]
  8. Gariglio P., Llopis R., Oudet P., Chambon P. The template of the isolated native simian virus 40 transcriptional complexes is a minichromosome. J Mol Biol. 1979 Jun 15;131(1):75–105. doi: 10.1016/0022-2836(79)90302-4. [DOI] [PubMed] [Google Scholar]
  9. Hentschel C. C., Tata J. R. Differential activation of free and template-engaged RNA polymerase I and II during the resumption of development of dormant Artemia gastrulae. Dev Biol. 1977 Jun;57(2):293–304. doi: 10.1016/0012-1606(77)90216-0. [DOI] [PubMed] [Google Scholar]
  10. Hentschel C. C., Tata J. R. Template-engaged and free RNA polymerases during Xenopus erythroid cell maturation. Dev Biol. 1978 Aug;65(2):496–507. doi: 10.1016/0012-1606(78)90044-1. [DOI] [PubMed] [Google Scholar]
  11. Kedinger C., Gniazdowski M., Mandel J. L., Jr, Gissinger F., Chambon P. Alpha-amanitin: a specific inhibitor of one of two DNA-pendent RNA polymerase activities from calf thymus. Biochem Biophys Res Commun. 1970 Jan 6;38(1):165–171. doi: 10.1016/0006-291x(70)91099-5. [DOI] [PubMed] [Google Scholar]
  12. Laub O., Jakobovits E. B., Aloni Y. 5,6-dichloro-1-beta-ribofuranosylbenzimidazole enhances premature termination of late transcription of simian virus 40 DNA. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3297–3301. doi: 10.1073/pnas.77.6.3297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mathis D., Oudet P., Chambon P. Structure of transcribing chromatin. Prog Nucleic Acid Res Mol Biol. 1980;24:1–55. doi: 10.1016/s0079-6603(08)60670-4. [DOI] [PubMed] [Google Scholar]
  14. Matsui S. I., Weinfeld H., Sandberg A. A. Quantitative conservation of chromatin-bound RNA polymerases I and II in mitosis. Implications for chromosome structure. J Cell Biol. 1979 Feb;80(2):451–464. doi: 10.1083/jcb.80.2.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Miller G., Berlowitz L., Regelson W. Chromatin and histones in mealy bug cell explants: activation and decondensation of facultative heterochromatin by a synthetic polyanion. Chromosoma. 1971;32(3):251–261. doi: 10.1007/BF00284838. [DOI] [PubMed] [Google Scholar]
  16. Tamm I., Kikuchi T. Early termination of heterogeneous nuclear RNA transcripts in mammalian cells: accentuation by 5,6-dichloro 1-beta-D-ribofuranosylbenzimidazole. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5750–5754. doi: 10.1073/pnas.76.11.5750. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weintraub H., Groudine M. Chromosomal subunits in active genes have an altered conformation. Science. 1976 Sep 3;193(4256):848–856. doi: 10.1126/science.948749. [DOI] [PubMed] [Google Scholar]
  18. Wu G. J. Adenovirus DNA-directed transcription of 5.5S RNA in vitro. Proc Natl Acad Sci U S A. 1978 May;75(5):2175–2179. doi: 10.1073/pnas.75.5.2175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Zentgraf H., Scheer U., Franke W. W. Characterization and localization of the RNA synthesized in mature avian erythrocytes. Exp Cell Res. 1975 Nov;96(1):81–95. doi: 10.1016/s0014-4827(75)80040-1. [DOI] [PubMed] [Google Scholar]
  20. Zurawski G., Brown K., Killingly D., Yanofsky C. Nucleotide sequence of the leader region of the phenylalanine operon of Escherichia coli. Proc Natl Acad Sci U S A. 1978 Sep;75(9):4271–4275. doi: 10.1073/pnas.75.9.4271. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. van der Westhuyzen D. R., Boyd M. C.D., Fitschen W., von Holt C. DNA-Dependent RNA polymerase in maturing avian erythrocytes. FEBS Lett. 1973 Mar 1;30(2):195–198. doi: 10.1016/0014-5793(73)80650-7. [DOI] [PubMed] [Google Scholar]

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