TA spectra of the charge-separated state [Re(bpy•−)]–•F3Y–βC19 collected 25 ns (top), 75 ns (middle) and 125 ns (bottom) after a 7 ns excitation pulse of laser light, λexc = 355 nm. Lifetime decays of the transient spectrum were measured at the four wavelengths indicated by the dashed lines: λdet at 380 and 480 nm correspond to the 3MLCT of [ReI]; 28 λdet = 525 nm is that of bpy•−;29 and, λdet = 425 nm is that of •F3Y. The solution contained 50 μM [Re]–F3Y–βC19 with 1 mM CDP, 3 mM ATP, 15 mM MgSO4, and 5% glycerol in 50 mM sodium borate (pH 8.3).