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SDS-PAGE, zymogram, immunoblot analysis, deglycosylation and cleavage by papain of purified recombinant rCDH. 1, pre-stained molecular weight marker; 2, 10 μg of purified CDH; 3, CDH zymogram on the sample 2; 4, Western blot analysis using anti-His antibodies; 5, CDH deglycosylated; 6, CDH digested by papain.
