Table 2.
Construct | Associativity | Mw/kD | Mn/kD | Mw/Mn | Rg/Å | Rh/Å | Rg/Rh |
---|---|---|---|---|---|---|---|
BclXL_dTM | Monomer | 28 ± 2 | 28 ± 2 | 1.00 ± 0.00 | ND | 33 ± 2 | ND |
Dimer | 54 ± 2 | 54 ± 2 | 1.00 ± 0.01 | ND | 41 ± 3 | ND | |
BclXL_FL | Monomer | 31 ± 1 | 31 ± 1 | 1.00 ± 0.01 | ND | 36 ± 1 | ND |
Dimer | 62 ± 3 | 61 ± 3 | 1.01 ± 0.01 | ND | 47 ± 2 | ND | |
Multimer | 361 ± 26 | 321 ± 25 | 1.14 ± 0.02 | 107 ± 4 | 99 ± 2 | 1.10 ± 0.01 | |
Polymer | 3355 ± 188 | 3134 ± 147 | 1.17 ± 0.02 | 198 ± 8 | 175 ± 6 | 1.13 ± 0.02 |
All parameters were obtained from ALS measurements at pH 8 and 10°C. Note that the calculated molar masses of recombinant BclXL_dTM and BclXL_FL constructs from their respective amino acid sequences are 27kD and 31kD, respectively. Errors were calculated from at least three independent measurements. All errors are given to one standard deviation. Note that the Rg parameter could not be determined (ND) for various species due to their lack of angular-dependence of scattered light.