. Author manuscript; available in PMC: 2013 Feb 10.

Published in final edited form as: J Mol Biol. 2011 Dec 14;416(1):57–77. doi: 10.1016/j.jmb.2011.12.015

Table 2.

Comparison of hydrodynamic parameters for BCLXL_dTM and BclXL_FL constructs

Construct	Associativity	M_w/kD	M_n/kD	M_w/M_n	R_g/Å	R_h/Å	R_g/R_h
BclXL_dTM	Monomer	28 ± 2	28 ± 2	1.00 ± 0.00	ND	33 ± 2	ND
BclXL_dTM	Dimer	54 ± 2	54 ± 2	1.00 ± 0.01	ND	41 ± 3	ND
BclXL_FL	Monomer	31 ± 1	31 ± 1	1.00 ± 0.01	ND	36 ± 1	ND
	Dimer	62 ± 3	61 ± 3	1.01 ± 0.01	ND	47 ± 2	ND
	Multimer	361 ± 26	321 ± 25	1.14 ± 0.02	107 ± 4	99 ± 2	1.10 ± 0.01
	Polymer	3355 ± 188	3134 ± 147	1.17 ± 0.02	198 ± 8	175 ± 6	1.13 ± 0.02

All parameters were obtained from ALS measurements at pH 8 and 10°C. Note that the calculated molar masses of recombinant BclXL_dTM and BclXL_FL constructs from their respective amino acid sequences are 27kD and 31kD, respectively. Errors were calculated from at least three independent measurements. All errors are given to one standard deviation. Note that the R_g parameter could not be determined (ND) for various species due to their lack of angular-dependence of scattered light.