Skip to main content
. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Dec 27;417(4):1188–1192. doi: 10.1016/j.bbrc.2011.12.105

Figure 2. Ankyrin deficiency does not affect cell surface TER119 expression or erythroblast enucleation in vitro.

Figure 2

(A) Analysis of mouse fetal erythroblast enucleation during in vitro culture. E13.5 fetal liver cells with the indicated genotype were stained with a biotinylated anti-TER119 monoclonal antibody. TER119 negative erythroid progenitor cells were purified and cultured in vitro in fibronectin-coated plates in medium containing serum and erythropoietin (Epo). The enucleation status of the cultured cells after two days of culture was analyzed by flow cytometric analysis using Hoechst 33342 and PE-TER-119. The percentage of enucleated cells is indicated. (B) Statistical analysis of the extent of enucleation in vitro in wild type, nb/+, and nb/nb mice.