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. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: Neuroscience. 2011 Dec 2;202:99–107. doi: 10.1016/j.neuroscience.2011.11.047

Figure 2. Subcellular analysis of polycystin-2 immunoreactivity in adult mouse RGCs.

Figure 2

A. Representative intensity analysis along a region of interest (ROI) line indicated in the bright field (BF) image. Intensity of immunoreactivity is plotted as 8 point moving average over distance (in μm). Arrows define the borders of the RGC as identified as peaks in BF intensity. Maximum intensity of polycystin-2 (PC-2) is in the cytoplasm, whereas Brn3a immunoreactivity co-localizes with the nuclear stain DAPI. B. A total of 89.8 ± 5.4% of cells in the RGC layer (defined based on positive DAPI staining) were positive for polycystin-2, whereas only 73.7 ± 2.8% of cells were positive for Brn3a (n=4, P<0.05). Quantification is based on analysis of maximum projection images of z-stacks (7–10 μm thickness) obtained from four different retinae.