(A) αβT cells. Proliferative responses were measured by CFSE dye dilution of primary αβT cells from Quad-deficient.Bcl2 (Quad-KO.Bcl2) mice against various stimulators. Data are representative of two experiments.
(B) CD155 epitopes recognized by MHC-independent TCRs. Schematic representation of chimeric mouse-human CD155 molecules are displayed (left). IL-2 response of TCR-transduced 4G4 cells (4×104) stimulated with 293T cells transfected with cDNA encoding various CD155 chimeric constructs (right). Each data point represents the mean (±SEM) of triplicate culture. Data are representative of two experiments.
(C) IL-2 response of TCR transduced 4G4 cells (1×105) stimulated with either native or heat denatured recombinant mCD155 protein. Each data point represents the mean (±SEM) of triplicate culture. Data are representative of three independent experiments.