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. Author manuscript; available in PMC: 2013 Jan 27.
Published in final edited form as: Immunity. 2011 Dec 29;36(1):79–91. doi: 10.1016/j.immuni.2011.11.013

Figure 6. Ligand Recognition by A11 and B12A TCR.

Figure 6

(A) Ligand recognition by A11 and B12A TCRs requires specific αβ pairing. IL-2 responses of 4G4 cells (4×104) transduced with the indicated TCR chains. Each data point represents the mean (±SEM) of triplicate culture. Data are representative of two experiments.

(B) Importance of CDR3α-encoded TCR sequences for CD155 recognition. CDR3α sequences were exchanged between B12A and B12F to generate chimeric F-CDR3-A and A-CDR3-F TCRα chains. F-CDR3-A designates B12F TCRα chains with B12A CDR3α sequences, and A-CDR3-F designates B12A TCRα chains with B12F CDR3α sequences. The indicated TCRα chains were transduced together with B12 TCRβ chains into 4G4 cells, and the TCR-transduced 4G4 cells (1×105) were stimulated with mCD155 (10 μg/ml). Each data point represents the mean (±SEM) of triplicate culture. Data are representative of three experiments.

(C) Role of CDR2β-encoded Y46, Y48, E54 TCR residues in mCD155 recognition. Y46, Y48, E54 amino acid residues in the TCR-Vβ8.1 chain of the A11 TCR were each mutated to alanine, were transduced into 4G4 cells along with the A11 TCRα chain, and stimulated with mCD155 protein (10 μg/ml). Each data point represents the mean (±SEM) of triplicate culture. Data are representative of three experiments.