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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Biochem Parasitol. 2011 Oct 15;181(2):94–101. doi: 10.1016/j.molbiopara.2011.10.004

Figure 3. Verification of the TbENF-TbTFIIB interaction using GST-TbENF.

Figure 3

(A) GST-TbENF interacting proteins were captured through incubation with procyclic T. brucei whole cell extract and the interactions were challenged with up to 0.9 M KCl washes (three 5 minute washes at room temperature). Interacting proteins were queried through Western blot. Using antibodies against TbTFIIB, TbRNAPII, and TbTBP, it was found that GST-TbENF interacts tightly with TbTFIIB (top panels), but not with TbRNAPII or TbTBP (bottom panels) under these conditions. (B) As a control, the GST-TbENF TbTFIIB interaction studies were carried out with T. brucei whole cell extract treated with DNase.