Figure 4. TbENF is a nuclear protein.
The nuclei (N) and cytoplasm (C) of procyclic T. brucei were fractionated through a 0.8 M sucrose cushion. TbENF was found to fractionate with TbTBP and TbTFIIB. Elongation factor 2 (EF-2) served as the cytoplasmic control, with the protein only observed in the cytoplasmic fraction.