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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Mol Biochem Parasitol. 2011 Oct 15;181(2):94–101. doi: 10.1016/j.molbiopara.2011.10.004

Figure 5. RNA interference studies reveal TbEFN49 is essential.

Figure 5

(A) Growth curves for clonal cell lines in which RNAi against TbENF was tetracycline induced (open squares) or non-induced (closed circles) in SDM-79 media. Parasite density, determined daily, is presented on a log scale. (B) Western blot analysis of TbENF levels in the RNAi-induced and non-induced cell lines. Each lane of the 10% SDS-PAGE gels contained 8×106 parasites. (C) Semiquantitative PCR to assess TbENF, Spliced Leader, and U6 RNA transcript levels on Day 4 of the RNA interference experiment.