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IEG activation: SaOS-2 cells received basal (B), five bouts of turbulent fluid flow (F) or 10 min of substrate strain (S) prior to mRNA extraction and real time PCR for IEG. ⁎=P<0.05, (Mann Whitney test and Kruskal Wallis test). N=3–4 per condition per experiment, with 3 independent repeat experiments.
