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. 1981 Jun 25;9(12):2717–2725. doi: 10.1093/nar/9.12.2717

16S ribosomal RNA of Escherichia coli contains a N2-methylguanosine at 27 nucleotides from the 3' end.

R Van Charldorp, H A Heus, P H Van Knippenberg
PMCID: PMC326887  PMID: 6792595

Abstract

The 49 nucleotides fragment derived from the 3' end of 16S rRNA by cloacin DF13, is not cleaved by ribonuclease T1 at a guanosine residue tha is present at 27 nucleotides from the 3' terminus (position 115 in 16S rRNA). Analysis of the isolated nucleotide indicates that it is a modified G residue. In vivo labeling with (3H)methionine shows that this G is methylated and co-chromatography with markers reveals that it is N2-methylguanosine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alberty H., Raba M., Gross H. J. Isolation from rat liver and sequence of a RNA fragment containing 32 nucleotides from position 5 to 36 from the 3' end of ribosomal 18S RNA. Nucleic Acids Res. 1978 Feb;5(2):425–434. doi: 10.1093/nar/5.2.425. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Azad A. A., Deacon N. J. The 3'-terminal primary structure of five eukaryotic 18S rRNAs determined by the direct chemical method of sequencing. The highly conserved sequences include an invariant region complementary to eukaryotic 5S rRNA. Nucleic Acids Res. 1980 Oct 10;8(19):4365–4376. doi: 10.1093/nar/8.19.4365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baan R. A., Hilbers C. W., Van Charldorp R., Van Leerdam E., Van Knippenberg P. H., Bosch L. High-resolution proton magnetic resonance study of the secondary structure of the 3'-terminal 49-nucleotide fragment of 16S rRNA from Escherichia coli. Proc Natl Acad Sci U S A. 1977 Mar;74(3):1028–1031. doi: 10.1073/pnas.74.3.1028. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baan R. A., van Charldorp R., van Leerdam E., van Knippenberg P. H., Bosch L., de Rooij J. F., van Boom J. H. The 3'-terminus of 16 S ribosomal RNA of Escherichia coli. Isolation and purification of the terminal 49-nucleotide fragment at a milligram scale. FEBS Lett. 1976 Dec 1;71(2):351–355. doi: 10.1016/0014-5793(76)80968-4. [DOI] [PubMed] [Google Scholar]
  5. Baer R., Dubin D. T. The 3'-terminal sequence of the small subunit ribosomal RNA from hamster mitochondria. Nucleic Acids Res. 1980 Nov 11;8(21):4927–4941. doi: 10.1093/nar/8.21.4927. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bowman C. M., Dahlberg J. E., Ikemura T., Konisky J., Nomura M. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo. Proc Natl Acad Sci U S A. 1971 May;68(5):964–968. doi: 10.1073/pnas.68.5.964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Brosius J., Palmer M. L., Kennedy P. J., Noller H. F. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4801–4805. doi: 10.1073/pnas.75.10.4801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Carbon P., Ehresmann C., Ehresmann B., Ebel J. P. The complete nucleotide sequence of the ribosomal 16-S RNA from Excherichia coli. Experimental details and cistron heterogeneities. Eur J Biochem. 1979 Oct 15;100(2):399–410. doi: 10.1111/j.1432-1033.1979.tb04183.x. [DOI] [PubMed] [Google Scholar]
  9. Donis-Keller H., Maxam A. M., Gilbert W. Mapping adenines, guanines, and pyrimidines in RNA. Nucleic Acids Res. 1977 Aug;4(8):2527–2538. doi: 10.1093/nar/4.8.2527. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Fellner P., Sanger F. Sequence analysis of specific areas of the 16S and 23S ribosomal RNAs. Nature. 1968 Jul 20;219(5151):236–238. doi: 10.1038/219236a0. [DOI] [PubMed] [Google Scholar]
  11. Helser T. L., Davies J. E., Dahlberg J. E. Change in methylation of 16S ribosomal RNA associated with mutation to kasugamycin resistance in Escherichia coli. Nat New Biol. 1971 Sep 1;233(35):12–14. doi: 10.1038/newbio233012a0. [DOI] [PubMed] [Google Scholar]
  12. Jordan B. R., Latil-Damotte M., Jourdan R. Sequence of the 3'-terminal portion of Drosophila melanogaster 18 S rRNA and of the adjoining spacer: comparison with corresponding prokaryotic and eukaryotic sequences. FEBS Lett. 1980 Aug 11;117(1):227–231. doi: 10.1016/0014-5793(80)80951-3. [DOI] [PubMed] [Google Scholar]
  13. Poldermans B., Roza L., Van Knippenberg P. H. Studies on the function of two adjacent N6,N6-dimethyladenosines near the 3' end of 16 S ribosomal RNA of Escherichia coli. III. Purification and properties of the methylating enzyme and methylase-30 S interactions. J Biol Chem. 1979 Sep 25;254(18):9094–9100. [PubMed] [Google Scholar]
  14. Rubtsov P. M., Musakhanov M. M., Zakharyev V. M., Krayev A. S., Skryabin K. G., Bayev A. A. The structure of the yeast ribosomal RNA genes. I. The complete nucleotide sequence of the 18S ribosomal RNA gene from Saccharomyces cerevisiae. Nucleic Acids Res. 1980 Dec 11;8(23):5779–5794. doi: 10.1093/nar/8.23.5779. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Samols D. R., Hagenbuchle O., Gage L. P. Homology of the 3' terminal sequences of the 18S rRNA of Bombyx mori and the 16S rRNA of Escherchia coli. Nucleic Acids Res. 1979 Nov 10;7(5):1109–1119. doi: 10.1093/nar/7.5.1109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shine J., Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. doi: 10.1073/pnas.71.4.1342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stanley J., Vassilenko S. A different approach to RNA sequencing. Nature. 1978 Jul 6;274(5666):87–89. doi: 10.1038/274087a0. [DOI] [PubMed] [Google Scholar]
  18. Van Charldorp R., Heus H. A., Van Knippenberg P. H. Adenosine dimethylation of 16S ribosomal RNA: effect of the methylgroups on local conformational stability as deduced from electrophoretic mobility of RNA fragments in denaturing polyacrylamide gels. Nucleic Acids Res. 1981 Jan 24;9(2):267–275. doi: 10.1093/nar/9.2.267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. de Graaf F. K., Niekus H. G., Klootwijk J. Inactivation of bacterial ribosomes in vivo and in vitro by cloacin DF13. FEBS Lett. 1973 Sep 1;35(1):161–165. doi: 10.1016/0014-5793(73)80601-5. [DOI] [PubMed] [Google Scholar]

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