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. 1981 Jun 25;9(12):2717–2725. doi: 10.1093/nar/9.12.2717

16S ribosomal RNA of Escherichia coli contains a N2-methylguanosine at 27 nucleotides from the 3' end.

R Van Charldorp, H A Heus, P H Van Knippenberg
PMCID: PMC326887  PMID: 6792595

Abstract

The 49 nucleotides fragment derived from the 3' end of 16S rRNA by cloacin DF13, is not cleaved by ribonuclease T1 at a guanosine residue tha is present at 27 nucleotides from the 3' terminus (position 115 in 16S rRNA). Analysis of the isolated nucleotide indicates that it is a modified G residue. In vivo labeling with (3H)methionine shows that this G is methylated and co-chromatography with markers reveals that it is N2-methylguanosine.

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Selected References

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