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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Mech Ageing Dev. 2011 Dec 13;133(1):30–36. doi: 10.1016/j.mad.2011.12.001

Fig. 2.

Fig. 2

Coomassie-blue-stained gels and Western blots of HNE- and MDA-modified proteins in mitochondria from skeletal muscles of 23-month-old C57BL/6 and DBA/2 mice, fed ad libitum (AL) or maintained on the calorically restricted (CR) diet since 4-months of age. Panels A and B are Coomassie blue-stained gels. Panels C, D, E and F are representative immunoblots of mitochondrial proteins reacting with anti-HNE or anti-MDA polyclonal antibodies. To confirm equal protein loading and provide for normalization, the membranes were re-probed using anti-porin antibody (shown at the bottom of the panels).