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. 2011 Nov 30;6(1):33–47. doi: 10.1016/j.molonc.2011.11.008

Figure 5.

Figure 5

uPA activates JAK2/STAT5 independent of GM‐CSF in HMEC. (A) Lysates were made from HMEC untreated or treated with pSV, puPA, purified uPA, siGM‐CSF, rhGM‐CSF, siGM‐CSF or uPA and immunoblotted for pJAK2, pSTA5, and total forms of JAK2 and STAT5 and with corresponding secondary antibodies. A representative blot of three independent experiments is shown. (B) Conditioned medium from untreated, control si, and siGM‐CSF treated HMEC was immunoblotted with primary antibody to GM‐CSF and corresponding secondary antibody. A representative blot of three independent experiments is shown. (C) Graph represents quantitative representation of the Western blotting results for three independent experiments, p<0.05. (D) Lysates were made from HMEC left untreated or treated with purified uPA, with rh GM‐CSF or both and immunoblotted for phospho and total forms of JAK2 and STAT5. A representative blot of three independent experiments is shown. (E) Graph represents quantitative expression of the Western blotting, p<0.05.