Figure 2. Increased Expression of mir-106b~25 in Cholangiocarcinoma Cell Lines and Human Tumor Samples.

Panel A: miR-106b expression was measured by qRT-PCT from total RNA isolated from lysates of the indicated cell lines. Results are presented as relative expression using Z30 as an internal control and employing the delta-delta Ct method (mean +/- SEM; n = 3; *p < 0.05, **p < 0.01). Panel B: miR-93 expression from indicated cell lines, presented as above (***p<0.001). Panel C: miR-25 expression from the indicated cell lines, presented as above. Panel D: Similarly, miR-106b was measured by qRT-PCR of total RNA isolated from frozen human tumor samples. Relative expression was determined (delta-delta Ct) compared to Z30 (internal control) and results are presented as fold change (log₂) compared to the average expression measured in four non-malignant human liver samples, and sorted based on expression. Sample numbers indicate the patients from which each was obtained. Panel E: miR-93 expression from human tumor cells, presented as above. Panel F: miR-25 expression from human tumors, presented as above.