Figure 4. Social space may depend on vision but not classical odor or cVA perception.

(A-C) Social Space Index is not modified in odor perception mutants; A) An allele of para (sbl¹) compared to genetic background Canton-s, Males, n=6 trials of ~40 flies. B) Or83b¹ and Or83b² were outcrossed 6 times and compared to genetic background Canton-s, n=6 trials of ~40 flies (SSI±SEM). C) Flies show less social aggregation in darkness, under a red light. Graph represents the comparison of Social Space Index in light and dark conditions (Males, n=15 trials of ~40 flies, t-test indicates a significant difference of p<0.005, **). D-F) Outcrossed mutants white disrupting the eye pigments localization show less social aggregation, normal geotaxis and reduced phototaxis. w¹¹¹⁸Cs₁₀ were outcrossed 10 times, indicated as w, compared to their genetic control Canton-s (Cs). D) Reduction in performance in the fast phototaxis assay. Flies were given 5 times 15 sec. to go towards the light, in a counter-current apparatus (n=8 trials of ~40 male flies; SSI±SEM – t-test indicates that Cs is different from w p<0.0006, ***). E) No differences in negative geotaxis. In a climbing assay in the counter-current apparatus, the flies were giving 15 sec. to reach the upper vial (n=5 trials of ~40 male flies (SSI±SEM) – t-test indicates no differences). F) Decreased SSI in males and females white. In males, n=21 trials for Cs, and n=18 trials for w (SSI±SEM); t-test indicates that Cs and w are different from w (p<0.05, * and p<0.001, ***). In females, n=16 trials for Cs, and n=12 trials for w; t-test indicates that w is different from Cs (p<0.001, ***).