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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Vascul Pharmacol. 2011 Oct 30;56(1-2):56–63. doi: 10.1016/j.vph.2011.10.002

Figure 5.

Figure 5

Effect of 2ME, taxol, and their co-administration on tubulin acetylation. A) HPAEC monolayers pretreated with vehicle control or 10 μM taxol for 30 min, and then challenged with vehicle control or 20 μM 2ME for 20 min were extracted and analyzed by Western blot with anti-acetyl-tubulin and anti-beta-tubulin antibodies. B) Lungs of mice subjected to intravenous administration of vehicle control, 10 mg/kg 2ME, 0.8 mg/kg taxol, and simultaneous administration of 2ME and taxol for 3h were extracted and analyzed with anti-acetyl-tubulin and anti-beta-tubulin antibodies. C) HPAEC monolayers grown on gold microelectrodes were challenged with vehicle control (circles) or 20 μM 2ME (squares) for the time indicated. TER was normalized to the point preceding 2ME addition; shown are mean±SEM (n=3). D) HPAEC monolayers challenged with vehicle control or 20 μM 2ME for the time indicated were extracted and analyzed with anti-acetyl-tubulin, anti-polyglutamylated tubulin, anti-tyrosinilated tubulin and anti-beta-actin antibodies.