Fig. 2.

Raptor is required for growth, but for not phagocytosis. (A,B) Raptor is required for growth. raptor RNAi cells were grown to log phase (<2×10⁶ cells/ml) in tetracycline and diluted ~10× into fresh media with or without tetracyline as indicated. Aliquots were taken at various times to evaluate (A) relative levels of endogenous raptor mRNA (~5 kb) by RNA blot analyses (rRNAs, ribosomal RNAs used as loading control markers) and (B) relative growth rates. (C) Raptor is not required for phagocytosis. raptor RNAi cells cultured with (control) or without tetracycline (raptor RNAi) for 34–40 hours. (see A,B) were assayed for relative phagocytosis by fluorimetric analyses. Arbitrary fluorescence units were used to normalize each strain relative to the maximum obtained for the control cells (plus tetracycline) within the same experiment. Three comparisons were made; values indicate means ± s.d.; differences to each other or to wild-type or mock cells (data not shown) were not significant. For each experiment, we first confirmed growth inhibition of raptor-depleted cells after removal of tetracycline (see B). Data are from cells that also displayed suppression of Raptor expression.