Fig. 8.

Delivery of recombinant periostin via electrospun collagen scaffolds recovers α-SMA expression in Postn^−/− mice. (A) Collagen type 1 electrospun scaffolds, with and without rhPN, were cut into 6 mm disks to match the size of punch wounds. (B) Periostin (green) protein was detected throughout the collagen + periostin scaffolds, but not the control collagen scaffolds. (C) Punch wounds were created in Postn^−/− animals and scaffolds were immediately laid into the wounds. Half of the wounds received two control (collagen only) scaffolds and the other half of the wounds received two collagen and periostin scaffolds. Wounds were harvested for IHC at day 7. Addition of periostin to Postn^−/− wounds by electrospun collagen scaffolds resulted in a marked increase in α-SMA immunoreactivity throughout the granulation tissue, when compared with that in wounds receiving control collagen scaffolds (n=3).