TABLE 2 .
Primers used for making FISH probesa
| PCR mix | Primer name | Orientation | Starting codon | Sequence from 5′ |
|---|---|---|---|---|
| egfp 1 | egfp-L31 | Forward | 1 | ATGGTGATGCGGTTTTGG |
| egfp 1 | egfp-R263 | Reverse | 232 | TAGCGGATCTGACGGTTC |
| egfp 1 | egfp-L287 | Forward | 256 | GCTCAAGCTTCGAATTCTGC |
| egfp 1 | egfp-R595 | Reverse | 564 | TCGTGCTGCTTCATGTGG |
| egfp 1 | egfp-L598 | Forward | 567 | TCTTCAAGTCCGCCATGC |
| egfp 1 | egfp-R757 | Reverse | 726 | ATGTTGCCGTCCTCCTTG |
| egfp 2 | egfp-L807 | Forward | 776 | ATGGCCGACAAGCAGAAG |
| egfp 2 | egfp-R985 | Reverse | 954 | TTGGGGTCTTTGCTCAGG |
| egfp 2 | egfp-L1035 | Forward | 1004 | ATCACTCTCGGCATGGAC |
| egfp 2 | egfp-L1146 | Forward | 1115 | TCCCCCTGAACCTGAAAC |
| egfp 2 | egfp-R1297 | Forward | 1266 | GAGTTTGGACAAACCACAAC |
| egfp 2 | egfp-R1403 | Reverse | 1372 | GCCGATTTCGGCCTATTG |
| egfp 3 | egfp-L1393 | Forward | 1362 | CCGAAATCGGCAAAATC |
| egfp 3 | egfp-R1555 | Reverse | 1524 | GGTGATGGTTCACGTAGTGG |
| egfp 3 | egfp-L1566 | Forward | 1535 | TTTTTGGGGTCGAGGTG |
| egfp 3 | egfp-R1766 | Reverse | 1735 | CTGTAGCGGCGCATTAAG |
| egfp 3 | egfp-L1783 | Forward | 1752 | CTGTAGCGGCGCATTAAG |
| egfp 3 | egfp-R1923 | Reverse | 1892 | TGGTTCTTTCCGCCTCAG |
| lacZ 1 | lacZ-L54 | Forward | 1 | ATTCCGCCGATACTGACG |
| lacZ 1 | lacZ-R172 | Reverse | 118 | ATTCCGCCGATACTGACG |
| lacZ 1 | lacZ-L189 | Forward | 135 | AGCGGCTGATGTTGAACTG |
| lacZ 1 | lacZ-R342 | Reverse | 288 | ACTGCCGCCTGTTTTGAC |
| lacZ 1 | lacZ-L361 | Forward | 307 | TTCTTGCGGCCCTAATCC |
| lacZ 1 | lacZ-R547 | Reverse | 493 | GCAGCATCAGGGGAAAAC |
| lacZ 2 | lacZ-L690 | Forward | 636 | CGCCAATGTCGTTATCCAG |
| lacZ 2 | lacZ-R910 | Reverse | 856 | TCTGGCGGAAAACCTCAG |
| lacZ 2 | lacZ-L1048 | Forward | 994 | TTGTGGAGCGACATCCAG |
| lacZ 2 | lacZ-R1226 | Reverse | 1172 | CGCTGACGGAAGCAAAAC |
| lacZ 2 | lacZ-L1286 | Forward | 1232 | GGCGTATCGCCAAAATCAC |
| lacZ 2 | lacZ-R1442 | Reverse | 1388 | ACAGTCTTGGCGGTTTCG |
| lacZ 3 | lacZ-L1453 | Forward | 1399 | GTGGGCGTATTCGCAAAG |
| lacZ 3 | lacZ-R1656 | Reverse | 1602 | CGCTGGATCAAATCTGTCG |
| lacZ 3 | lacZ-L1659 | Forward | 1605 | ACAGCGCGTCGTGATTAG |
| lacZ 3 | lacZ-R1816 | Reverse | 1762 | CATGGTGCCAATGAATCG |
| lacZ 3 | lacZ-L1893 | Forward | 1839 | TCGGATAATGCGAACAGC |
| lacZ 3 | lacZ-R2071 | Reverse | 2017 | GGTGCGGATTGAAAATGG |
| lacZ 4 | lacZ-L2086 | Forward | 2032 | ATCGCAGGCTTCTGCTTC |
| lacZ 4 | lacZ-R2252 | Reverse | 2198 | TCGGCGGTGAAATTATCG |
| lacZ 4 | lacZ-L2278 | Forward | 2224 | CGTTTCACCCTGCCATAAAG |
| lacZ 4 | lacZ-R2448 | Reverse | 2394 | TTCCGTGACGTCTCGTTG |
| lacZ 4 | lacZ-L2457 | Forward | 2403 | TCATCCGCCACATATCCTG |
| lacZ 4 | lacZ-R2556 | Reverse | 2502 | CTGAGCGCATTTTTACGC |
| lacZ 5 | lacZ-L2564 | Forward | 2510 | TCAGACGGCAAACGACTG |
| lacZ 5 | lacZ-R2734 | Reverse | 2680 | TGTTCCCACGGAGAATCC |
| lacZ 5 | lacZ-L2739 | Forward | 2685 | GCGGATTGACCGTAATGG |
| lacZ 5 | lacZ-R2917 | Reverse | 2863 | CAGCCTGAATGGCGAATG |
| lacZ 5 | lacZ-L2916 | Forward | 2862 | TGCGCAACTGTTGGGAAG |
| lacZ 5 | lacZ-R3057 | Reverse | 3003 | TCACTGGCCGTCGTTTTAC |
a
The sequences of the PCR primers used for generating the FISH probes are listed. The position and orientation of each probe relative to the first probe for each gene are given.